Agilent HPLC

usPmethod

 usPPackingmaterials

 Column

Particlesize(µm)

Poresize(Å)

L1

Octadecyl silane chemically bonded to porous silica or ceramic

Poroshell 120 EC-C18

2.7

120

microparticles, 1.5 to 10 µm in diameter, or a monolithic rod

Poroshell 120 SB-C18

2.7

120

Poroshell 300SB-C18

5

300

Poroshell 300 Extend-C18

5

300

ZORBAX Eclipse Plus C18

1.8, 3.5, 5

95

ZORBAX Eclipse XDB-C18

1.8, 3.5, 5, 7

80

ZORBAX StableBond SB-C18

1.8, 3.5, 5, 7

80, 300

ZORBAX Rx-C18

3.5, 5

80

ZORBAX Extend-C18

1.8, 3.5, 5, 7

80, 300

ZORBAX ODS

3, 5, 7

70

ZORBAX ODS classic

5

70

Pursuit XRs C18

3, 5, 10

100

Pursuit C18

3, 5, 10

200

Pursuit C18-A

3, 5, 10

180

Polaris C18-Ether

3, 5

200

SepTech ST60 C18

10

60

SepTech ST150 C18

10

150

Agilent Prep C18

5, 10

100

L3

Porous silica particles, 1.5 to 10 µm in diameter, or a monolithic silica

ZORBAX HILIC Plus

1.8, 3.5

95

rod

ZORBAX SIL

5

70

ZORBAX Rx-SIL

3.5, 5, 7

80, 300

Pursuit XRs Si

3, 5, 10

100

Polaris Si-A

5, 10

180

Agilent Prep

5, 10

100

L7

Octylsilane chemically bonded to totally porous silica particles, 1.5 to 10

Poroshell 120 EC-C8

2.7

120

µm in diameter, or a monolithic silica rod

Poroshell 120 SB-C8

2.7

120

Poroshell 300SB-C8

5

300

ZORBAX Eclipse Plus C8

1.8, 3.5, 5

95

ZORBAX Eclipse XDB-C8

1.8, 3.5, 5, 7

80

ZORBAX SB-C8

1.8, 3.5, 5, 7

80, 300

ZORBAX Rx-C8

1.8, 3.5, 5, 7

80

ZORBAX C8

5

70

Pursuit XRs C8

3, 5, 10

100

Pursuit C8

3, 5, 10

200

Polaris C8-A

3, 5

180

Polaris C8-Ether

3, 5

200

L8

An essentially monomolecular layer of aminopropylsilane chemically

ZORBAX NH2

5

70

bonded to totally porous silica gel support, 3 to 10 µm in diameter

Polaris NH2

5

180

L9

Irregular or spherical, totally porous silica gel having a chemically bonded, strongly acidic cation-exchange coating, 3 to 10 µm in diameter

ZORBAX SCX

5 spherical

300

L10

Nitrile groups chemically bonded to porous silica particles,

ZORBAX CN

5

70

3 to 10 µm in diameter

ZORBAX SB-CN

3.5, 5

80, 300

ZORBAX Eclipse XDB-CN

3.5, 5

80

L11

Phenyl groups chemically bonded to porous silica particles,

ZORBAX Eclipse XDB Phenyl

5

70

1.5 to 10 µm in diameter

ZORBAX Eclipse Plus Phenyl-Hexyl

1.8, 3.5, 5

95

ZORBAX Phenyl

3.5

80

Poroshell 120 Phenyl-Hexyl

2.7

120

Pursuit XRs DiPhenyl

3, 5, 10

100

Pursuit DiPhenyl

3, 5, 10

200

L13

Trimethylsilane chemically bonded to porous silica particles, 3 to 10 µm in diameter

ZORBAX TMS

5

70

L14

Silica gel having a chemically bonded, strongly basic quaternary

ZORBAX SAX

5

70

ammonium anion-exchange coating, 5 to 10 µm in diameter

IonoSpher A

5

120

L17

Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the hydrogen form, 7 to 11 µm in diameter

Hi-Plex H

8

N/A

L19

Strong cation-exchange resin consisting of sulfonated cross-linked

Hi-Plex Ca

8

N/A

styrene-divinylbenzene copolymer in the calcium form, 9 µm in diameter

Hi-Plex Ca (Duo)

8

N/A

L20

Dihydroxypropane groups chemically bonded to porous silica particles, 3 to 10 µm in diameter

LiChrospher Diol

5

N/A

L21

A rigid spherical styrenedivinylbenzene copolymer,

PLRP-S

3, 5, 8, 10, 10-

100

5 to 10 µm in diameter

15, 15-20, 50

PLRP-S

3, 5, 8, 10, 10-

300

15, 15-20, 50

PLRP-S

5, 8, 10, 30, 50

1000

PLRP-S

5, 8, 10, 30, 50

4000

PLgel

3, 5, 10, 20

50, 100, 500,

103, 105,

105, 106,

MIXED

L22

A cation-exchange resin made of porous polystyrene gel

Hi-Plex H

8

N/A

with sulfonic acid groups, about 10 µm in size

L25

Packing having the capacity to separate compounds with a MW range

PL aquagel-OH

5, 8

30

from 1,000 to 5,000 da (as determined by the polyethylene oxide),

applied to neutral, anionic and cationic water-soluble polymers. A

polymethacrylate resin base, crosslinked with polyhydroxylated ether

(surface contained some residual carboxyl functional gruops) was

found suitable

L33

Packing having the capacity to separate dextrons by molecular size

over a range of 4,000 to 500,000 da. It is spherical, silica-based,

and processed to provide pH stability

ZORBAX GF-250

4

150

Bio SEC-3

3

100, 150,

300

5

100, 150,

300, 500,

1000, 2000

5

300